β-glucan, a polysaccharide known for its distinctive molecular structure and diverse biological activities, holds a significant position in various industrial applications. Abundantly present in sources such as oats, barley, mushrooms, and yeast, β-glucan is renowned for its versatility across sectors including foods, medicines, cosmetics, feeds, and health products. In this chapter, an exploration is undertaken to investigate the varied industrial applications of β-glucan, a widely recognized polysaccharide with multifaceted properties. More research is needed to exploit the potential of employing β-glucan to enhance product quality, functionality, and overall consumer well-being.