Chronic wounds, particularly those associated with diabetes and peripheral arterial disease, present significant clinical and economic challenges, often resulting in limb amputations. Laserobaria 2.0_S is a multifunctional therapeutic device integrating Topical Oxygen Therapy (TOT), ozone therapy, Pulsed Electromagnetic Field (PEMF) therapy, and Low-Level Light Therapy (LLLT) to accelerate wound healing and reduce inflammation in the limbs area. It is the world’s first and only device that integrates five physical factors within a single wound healing support system. The device was developed based on the Laserobaria-S prototype, where therapy resulted in a 36.4–40.5% reduction in ulcer size and significant pain relief (p<0.05). The evaluation of Laserobaria 2.0_S in clinical settings involving 52 patients demonstrated an improvement in wound healing in 40.5% of cases and a reduction in pain in 60%. Additionally, a 25% decrease in C-reactive protein (CRP) levels and an 18% reduction in fibrinogen were observed, confirming the therapy’s anti-inflammatory effects. This study aims to assess the impact of physical factors on regenerative processes. The retrospective analysis focuses on verifying the effectiveness of the applied factors in tissue regeneration, by comparing pilot and observational studies conducted in a clinical setting. Such analysis serves to therapy optimization is also crucial due to the potential synergistic effects between physical factors, which may enhance therapeutic outcomes. The findings obtained from the proposed analysis will contribute to further research on the standardization of therapeutic protocols and the optimization of device performance conditions to achieve the highest treatment effectiveness.