Mass timber composites (MTCs) have gained interest for their potential to achieve long floor spans. This paper investigates the experimental performance of three screw-glued shear connection configuration for use in MTCs. The configurations investigated used different screws and clamping pressure to study the influence on the connection stiffness, strength and failure modes. A commonly available construction adhesive shown to have adequate performance is used in the connection. The clamping pressure varied from 0.19 to 0.28 MPa, using washer head or double threaded self-tapping screws. For each of the load-slip curves, three bilinear data fit models for evaluating the ductility of timber connections are used to estimate the transition point between the near-linear initial behaviour and the non-linear strain hardening behaviour. This is deemed to be the connection yield point. Overall, the results showed that the connection capacity and stiffness were comparable for all configurations with average initial stiffnesses of 14.50–16.62 N/mm per mm2 of adhesive and max shear stresses of 4.17–5.20 MPa. The amount of wood failure in the shear plane post-test varied across specimens (30–100%); however, those showing more glue failure did not exhibit lower stiffnesses or capacities than the full-wood failure specimens. This indicated that the adhesive was adequate at filling any gaps in the bonding surface, and the quality of wood generally govern the behaviour. Overall, the screw-glued connections showed excellent performance and can be deemed a promising and economical solution for shear connectors in MTCs.