This paper focuses on the cultivation of talents in artificial intelligence (AI) technology applications in higher vocational education. It analyzes the existing issues in the context of industry-education integration, constructs a talent cultivation system that seamlessly connects “science, education, and industry,” elaborates on its implementation pathways and effectiveness, and proposes innovative strategies and outlooks. The aim is to provide a comprehensive and in-depth theoretical and practical reference for the cultivation of AI talents in higher vocational education, promoting innovation in talent cultivation models and synergistic progress with industrial development.