Previous studies have shown that providing donation education through smartphone applications (apps) significantly enhances knowledge and improves users’ attitudes. In this study, two hundred forty participants were surveyed to determine the perceived persuasiveness of the Eye Donor Aust app. SPSS software was employed to test the correlations between demographic variables and analyze data distribution, and Structural Equation Modeling was created to explore the multivariate causal relationships among constructs. The findings indicate that three PSD principles (Credibility Support, Dialogue Support, and Social Support) significantly enhanced the app’s persuasiveness, while participants’ age and gender were significantly associated with the app’s persuasiveness. These findings can inform the development of high-quality educational apps employing PSD principles that effectively persuade users.