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Biomedical engineering is a discipline that integrates engineering and biological sciences to address healthcare challenges. It employs biomaterials and cell therapy to tailor treatments for tissue regeneration. Bioceramic materials play a crucial role in bone regenerative medicine, bonding with tissue, promoting bone growth and aiding in effective repair. Additionally, bioceramics enhance skin regeneration by stimulating fibroblasts, improving healing quality and efficiency through various of their properties. This study evaluated the physicochemical characteristics and biocompatibility of various bioceramics (BCs) for potential applications in bone and skin regeneration. Particles of 45S5, B1P, B2P, BG-2A, BG-2B, E1, F1 and G1 were characterized microscopically and by zeta potential. Cell viability was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) after 1 day of treatment. For this test, fibroblasts were seeded on 96-well tissue culture plates and treated with the active ceramics at a concentration of 0.5 mg/ml and volumes of 100 and 200μl. The results of optical microscopy analysis revealed irregular-shaped particles across the BC samples. The commercial 45S5 bioglass exhibited fine, pointed particles, while the B1P group displayed larger particles (>100 μm). The B2P, BG-2A, E1, F1 and G1 samples exhibited smaller sizes and a tendency to agglomerate. Zeta potential measurements indicated that the E1 sample demonstrated higher stability, as indicated by its highly negative zeta potential value of -40.83mV. The B2P, BG-2A, BG-2B, F1 and G1 samples exhibited zeta potential values close to −25 mV, reflecting their relative stability in suspension. The B1P and 45S5 samples displayed the lowest stability characteristics. The mitochondrial metabolism of the cells exposed to the BCs was assessed using the MTT assay, which demonstrated that there was no significant impact on cell viability after a 1-day exposure period. These findings indicate that the tested BCs have the potential of being utilized in regenerative medicine applications due to their favorable physicochemical properties and non-cytotoxic nature.

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Characterization and Comparison of the Effects of Bioactive Ceramics for Bone and Skin Regeneration

  • 会议论文

  • 发表日期：

    2025年

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  • J. Vaniel,
  • N. Maurmann,
  • R. Siqueira,
  • P. Pranke

摘要

Biomedical engineering is a discipline that integrates engineering and biological sciences to address healthcare challenges. It employs biomaterials and cell therapy to tailor treatments for tissue regeneration. Bioceramic materials play a crucial role in bone regenerative medicine, bonding with tissue, promoting bone growth and aiding in effective repair. Additionally, bioceramics enhance skin regeneration by stimulating fibroblasts, improving healing quality and efficiency through various of their properties. This study evaluated the physicochemical characteristics and biocompatibility of various bioceramics (BCs) for potential applications in bone and skin regeneration. Particles of 45S5, B1P, B2P, BG-2A, BG-2B, E1, F1 and G1 were characterized microscopically and by zeta potential. Cell viability was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) after 1 day of treatment. For this test, fibroblasts were seeded on 96-well tissue culture plates and treated with the active ceramics at a concentration of 0.5 mg/ml and volumes of 100 and 200μl. The results of optical microscopy analysis revealed irregular-shaped particles across the BC samples. The commercial 45S5 bioglass exhibited fine, pointed particles, while the B1P group displayed larger particles (>100 μm). The B2P, BG-2A, E1, F1 and G1 samples exhibited smaller sizes and a tendency to agglomerate. Zeta potential measurements indicated that the E1 sample demonstrated higher stability, as indicated by its highly negative zeta potential value of -40.83mV. The B2P, BG-2A, BG-2B, F1 and G1 samples exhibited zeta potential values close to −25 mV, reflecting their relative stability in suspension. The B1P and 45S5 samples displayed the lowest stability characteristics. The mitochondrial metabolism of the cells exposed to the BCs was assessed using the MTT assay, which demonstrated that there was no significant impact on cell viability after a 1-day exposure period. These findings indicate that the tested BCs have the potential of being utilized in regenerative medicine applications due to their favorable physicochemical properties and non-cytotoxic nature.