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An acoustofluidic chip designed for three-dimensional cell microculture is introduced in this work. Constructed from cost-effective materials such as plastic and glass, the chip features a microwell structure where cells are to be injected and cultivated. Through the action of an ultrasound standing wave engineered within the microwell, cells are induced to form stable aggregates, levitating in relation to the chip’s superstrate. Additionally, a background fluid flow can be established within the microwell to facilitate the delivery of nutrients and specific substances for interaction with the cells. The size of the aggregates, reaching more than 100 cells, is dependent on the initial cell concentration. Current challenges in optimizing this setup for practical and routine cell assay experiments are discussed.

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Toward Cell Microculture Using Acoustofluidics Technology

  • 会议论文

  • 发表日期：

    2025年

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  • Karen S. C. Borbely,
  • Flávio O. S. D’Amato,
  • Karoline S. Q. da Silva,
  • Deyvid W. S. França,
  • Ana Lúcia M. Silva,
  • Ana Flávia S. Lima,
  • Alexandre U. Borbely,
  • Glauber T. Silva

摘要

An acoustofluidic chip designed for three-dimensional cell microculture is introduced in this work. Constructed from cost-effective materials such as plastic and glass, the chip features a microwell structure where cells are to be injected and cultivated. Through the action of an ultrasound standing wave engineered within the microwell, cells are induced to form stable aggregates, levitating in relation to the chip’s superstrate. Additionally, a background fluid flow can be established within the microwell to facilitate the delivery of nutrients and specific substances for interaction with the cells. The size of the aggregates, reaching more than 100 cells, is dependent on the initial cell concentration. Current challenges in optimizing this setup for practical and routine cell assay experiments are discussed.