The Vero cell lineage, derived from the kidney of the African green monkey, plays an essential role in scientific research due to its ease of cultivation and viral resistance. A spheroid culture, a three-dimensional approach to cell culture, offers an innovative model for analyzing cell interaction, applicable not only to Vero cells but also to stem cell models and those showing tumor resistance. Biomaterials, whether natural or synthetic, are fundamental in biomedical applications, interacting with biological systems to treat, enhance, or replace tissues and organs. The concept of biomimicry emphasizes its active influence on recovery, including at the cellular level. This project aims to create and characterize Vero cell spheroids, with the aim of enabling the evaluation of their interaction with the organism, properties such as biocompatibility and cytotoxicity, as well as their utility in the biological assessment of biomaterials. Thus, it contributes to the understanding of spheroids as three-dimensional models and evaluates their applicability in biomedical research.