At the mesoscale, investigating the distribution of myocardial sheet thickness in laminar structures can help understand the relationship between myocardial function and sheet arrangement. The distance between the cleavage planes (DCP) is a metric to assess the myocardial dynamic rearrangement. In this paper, we investigated the arrangement of local 3D laminar structure in human left ventricle free-wall samples using X-ray phase-contrast tomography with an isotropic resolution of 3.5 µm. We extracted the multiscale laminar structures in each sample using a morphological method and calculated DCP values in typical small-sized volumes using the distance-transform method. The statistical results show the distribution profile of the DCP in one-sheet population and two-sheet populations of local regions, which indicates the complexity and locality of laminar structures.