Minimizing sampling bias and ensuring reproducibility in behavioral studies of aquatic species would depend strongly on an appropriate experimental design. Research questions often demand modifications, including innovative tank set-ups. The T-maze is a widely used design for testing color preference in zebrafish, and here, it was adapted to examine the effects of anthropogenic noise on decision-making. In this set-up, one arm (red) displayed a video of a moving zebrafish shoal, while the other (green) lacked a social stimulus. To prevent side bias, arm presentation was alternated between left and right. Individual zebrafish were trained across three consecutive trials under three treatments (Q–Q–Q, Q–Q–N, and N–N–N) to associate the red arm with the social stimulus. Decision latency and time spent in the social area were recorded. Results demonstrate that the T-maze is not only valid for testing color and side preference but also effective in assessing noise impacts on memory and decision-making.

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Using a T-Maze to Explore Noise Effects on Decision-Making in Freshwater Fish: A Methodological Approach

  • Zahra Samandar,
  • Anuradha Bhat,
  • Saeed Shafiei Sabet

摘要

Minimizing sampling bias and ensuring reproducibility in behavioral studies of aquatic species would depend strongly on an appropriate experimental design. Research questions often demand modifications, including innovative tank set-ups. The T-maze is a widely used design for testing color preference in zebrafish, and here, it was adapted to examine the effects of anthropogenic noise on decision-making. In this set-up, one arm (red) displayed a video of a moving zebrafish shoal, while the other (green) lacked a social stimulus. To prevent side bias, arm presentation was alternated between left and right. Individual zebrafish were trained across three consecutive trials under three treatments (Q–Q–Q, Q–Q–N, and N–N–N) to associate the red arm with the social stimulus. Decision latency and time spent in the social area were recorded. Results demonstrate that the T-maze is not only valid for testing color and side preference but also effective in assessing noise impacts on memory and decision-making.