The interactive mobile hospital guidance system has become increasingly pivotal in people's lives, significantly easing remote consultations for patients and reducingtime consumption. The visual interface design serves as the cornerstone of patient interaction. However, most current interactive mobile hospital navigation systems cater to the general population and overlook the exploration of elderly users' interaction experiences. This study delves into the impact of information presentationmode and map view presentation type on elderly users' user experience in interactivemobile hospital navigation systems. The study's variables encompassed information presentation types (i.e., icon + text and plain text) and map view presentation types (i.e., 2D and 3D). Using a 2 × 2 between-subjects design, a total of 40 participants were purposefully sampled and asked to complete questionnaires based on a 7-point Likert scale for subjective evaluation and semi-structured interviews. The results revealed the following: (1) When using the mobile hospital positioning interface, elderly users prefer the presentation of 2D maps with icons and have access to clearer information about landmarks; (2) Overall, the advantage of icons in the type of information presentation is not obvious in the mobile hospital positioning interface; (3) When the map is presented in a three-dimensional way, the icons increase the visual perceptual load of the elderly users in the mobile hospital positioning interface, affecting the the quickness of visual processing for elderly users, while the opposite is true when presented in a two-dimensional manner. This study provides a design reference for the future development of mobile hospital positioning interfaces for the elderly.