Toxicological expertise is an integral part of assessing the safety of any pharmacological agent and/or its pharmacological effects. The results directly influence the design of further studies, the choice of drug dosages, and the limitations regarding the target application group. Although behavioral and basic physiological parameters, such as pulse, respiration, temperature, behavioral changes, and mortality, are measured during such experiments, an objective evaluation of the functional state of organs and in vivo systems is equally important. In this context, the use of comprehensive abdominal ultrasonography in dynamic studies within the framework of preclinical research protocols represents a promising and informative method. It allows for a more detailed evaluation of the effects of pharmacological agents on tissues and organ systems. This article presents the results of ultrasound diagnostics performed within the protocol for subchronic toxicity testing of this new compound. We developed a feed supplement based on chromium, a biometal and an amino acid complex. This article presents the results of ultrasound diagnostics performed within the protocol for subchronic toxicity testing of this new compound. During the experiment, high potentially toxic doses of the veterinary supplement (1/10 of the maximum allowable dose) were administered. Structural and functional changes were observed, which were not detected during behavioral monitoring. These findings suggest that ultrasound diagnostics could serve as an essential and effective method for toxicological assessments.