International policies highlight the integration of digital technologies in education to foster skills in digital literacy, collaboration, and internationalization, positioning teacher education at the core of innovation. This exploratory study builds on a 2024 research project at the University of Foggia (Italy) to rethink Initial Teacher Training (ITT) through digital innovation and internationalization, using eTwinning as a transformative platform. In Italy, universities have increasingly promoted eTwinning’s integration into Initial Teacher Education (ITE) programs. The main objective was to assess eTwinning’s effectiveness in enhancing pre-service teachers’ competencies in managing innovative learning environments, focusing on digital and international collaboration. A mixed-methods approach was used, with 204 pre-service teachers completing a Computer Assisted Web Interviewing (CAWI) questionnaire, followed by the analysis of 39 technological artifacts created by participants. The results showed that eTwinning was valued for fostering collaboration, enhancing digital skills, and promoting international networking. Among the key challenges were technical difficulties, language barriers, and time management, while strengths included creativity, adaptability, and teacher support. The study emphasizes the importance of integrating eTwinning into ITT programs to enhance innovation and digital skills. It was noted that eTwinning not only promotes international collaboration but also encourages universities to rethink their training pathways by integrating AI, technological devices, and networking tools. It highlights the value of informal learning and a multi-level educational approach, urging universities to innovate and adapt their curricula and foster digital and cross-cultural competencies. The study’s findings highlight the potential of eTwinning to redefine ITT, offering a model that aligns with international trends in digital transformation and educational innovation. These findings contribute to international debates on teacher training, highlighting the need for strategically integrating digital technologies in education.