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This chapter addresses innovative green methodologies for the extraction of microalgal pigments, focusing on the integration and reduction of steps. Water and phosphate buffers have proven effective in extracting phycobiliproteins due to their ability to dissolve water-soluble compounds. However, for lipophilic pigments, such as chlorophylls and carotenoids, solvents like ethanol and deep eutectic solvents (DES) are preferred due to their solubilization capacity and lower toxicity compared to conventional organic solvents. The integration of these eco-friendly solvents with emerging technologies, such as ultrasound (US), microwave (MW), and pulsed electric field (PEF), has shown great potential in optimizing the extraction of these pigments by promoting cell disruption and intracellular content release. Supercritical CO₂ extraction also stands out as an effective approach for extracting lipophilic pigments. For phycobiliproteins, supercritical CO₂ can be applied as a pretreatment or in combination with higher polarity co-solvents. However, challenges related to scalability, cost, and efficiency still need to be overcome for the successful application of these microalgal pigment extraction processes.

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Novel Protocols for Microalgae Pigment Recovery: Simultaneous Extraction Approaches

  • 章节

  • 发表日期：

    2026年

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  • Raquel Guidetti Vendruscolo,
  • Larissa Rodrigues Gomes,
  • Hugo José Martins Carvalho

摘要

This chapter addresses innovative green methodologies for the extraction of microalgal pigments, focusing on the integration and reduction of steps. Water and phosphate buffers have proven effective in extracting phycobiliproteins due to their ability to dissolve water-soluble compounds. However, for lipophilic pigments, such as chlorophylls and carotenoids, solvents like ethanol and deep eutectic solvents (DES) are preferred due to their solubilization capacity and lower toxicity compared to conventional organic solvents. The integration of these eco-friendly solvents with emerging technologies, such as ultrasound (US), microwave (MW), and pulsed electric field (PEF), has shown great potential in optimizing the extraction of these pigments by promoting cell disruption and intracellular content release. Supercritical CO₂ extraction also stands out as an effective approach for extracting lipophilic pigments. For phycobiliproteins, supercritical CO₂ can be applied as a pretreatment or in combination with higher polarity co-solvents. However, challenges related to scalability, cost, and efficiency still need to be overcome for the successful application of these microalgal pigment extraction processes.