Objective. Τo investigate the effect of tailored HPV vaccine messages content on HPV vaccination intention in women aged 18–25 years. Methods Convenience sampling method was adopted, and a self-designed questionnaire titled Concerned HPV Vaccine Issues and Vaccination Intention was used to conduct the survey by online answering method. Based on the information needs of participants, they were divided into three groups: provided with no tailored messages (group A), provided with the most concerned messages (group B), and provided with all concerned messages (group C). The last two groups were set up to study the effect of tailored messages on vaccination intention. Results 267 women aged 18–25 who did not receive or did not complete the vaccination completed the questionnaire, at last we received 265 valid questionnaires and 2 invalid questionnaires. Among the 265 participants, 38 received no tailored messages, 123 received the most concerned messages, and 104 received all concerned messages. In group B, 97 (78.86%) concentrated on the effectiveness of the vaccine, 87 (70.73%) on its adverse reactions, and 57 (46.34%) on the messages about vaccine type and related diseases. After the tailored messages intervention, the vaccination intention of groups B and C was lower than that of group A, and providing the most concerned HPV vaccine messages had a more significant impact on the vaccination intention than providing all the concerned messages. A comparative analysis of the vaccination intentions among the three groups revealed a statistically significant difference (Z = 8.497, P < 0.05). Conclusion After the tailored messages intervention, the overall intention to vaccinate was decreased, which means information overload and anxiety might affect the intention to vaccinate. It is recommended that epidemic prevention agencies should increase the popularization of HPV vaccine and vaccine-related knowledge, and improve the correct cognition of at-risk groups on vaccines and vaccine-related knowledge.