In order to ensure the safe and efficient operation of the remote tower system, this paper further analyzes the workload of the controllers under different control modes. The selected experimental subjects were front-line controllers. The field tracking method was used to obtain the eye movement characteristic data and NASA-TLX scale data commanded by controllers under two control modes of traditional physical and remote tower. The results show that there are significant differences in the mean number of blinks, number of fixations, average blink duration, average fixation duration (P < 0.05). There are significant differences in mental demand, physical demand, time demand and total mental load (P < 0.05). By quantifying the workload value of the model, it is found that compared with traditional physical tower control, the workload of remote tower control is relatively increased. This study can provide reference for the follow-up study of human factor ergonomics in remote tower, and is of great significance to ensure the safety of civil aviation.