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Recently, ozone has been widely used in medicine and clinical practice. The ozonation of biological fluids also requires real-time analysis due to the fast reactions involved. Thus, we develop an innovative ozone sample holder of biological fluids monitored in real-time by spectroscopy. The ozone sample holder has a simple design and is hand-assembled, with a body in stainless steel and a porous PTFE membrane as a gas diffuser. Dye and urine were ozonated and the spectra collection was based on UV-visible and Raman spectroscopy, respectively. The device requires a small sample volume and shows a good spectra signal response through the real-time fluid ozonation, and in an interesting mass transfer process.

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A Sample Holder to Ozonation of Biological Fluids Monitored in Real-Time by Spectroscopic

  • 会议论文

  • 发表日期：

    2025年

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  • Henrique Cunha Carvalho,
  • Landulfo Silveira,
  • Renato Amaro Zângaro,
  • Adriana Barrinha Fernandes,
  • Leandro Procópio Alves,
  • Livia Helena Moreira,
  • Carlos José de Lima

摘要

Recently, ozone has been widely used in medicine and clinical practice. The ozonation of biological fluids also requires real-time analysis due to the fast reactions involved. Thus, we develop an innovative ozone sample holder of biological fluids monitored in real-time by spectroscopy. The ozone sample holder has a simple design and is hand-assembled, with a body in stainless steel and a porous PTFE membrane as a gas diffuser. Dye and urine were ozonated and the spectra collection was based on UV-visible and Raman spectroscopy, respectively. The device requires a small sample volume and shows a good spectra signal response through the real-time fluid ozonation, and in an interesting mass transfer process.