Bio-nanotechnology and medicine both depend significantly on nanotherapeutic compounds. Applications based on plant materials and silver nanoparticles (AgNP) have been approved for use in biomedicine. Plant-based nanoparticle synthesis has garnered significant attention due to its cost-effectiveness, sustainable nature, eco-friendliness, and array of applications. Scanning Electron Microscopy (SEM), and X-ray diffraction (XRD), were used to evaluate AgNPs made using Nigella sativa extract. The results indicated a spherical form and a peak at 430 nm in size. This work describes the environmentally friendly production of silver nanoparticles (AgNPs) with seed extract from Nigella sativa. An easy, affordable, and environmentally benign process was used to create the AgNPs. According to the findings, the Nigella sativa (NS) extract could convert Ag+ ions into AgNPs, which would produce a consistent and stable dispersion of spherically-shaped nanoparticles with an average size of 20–32 nm. Bioactive substances such flavonoids, alkaloids, and phenols that served as reducing and stabilizing agents for the AgNPs were proven to be present in the NS extract. With Nigella sativa extract, this work offers a sustainable and environmentally friendly method for synthesizing AgNPs that might be further investigated for a range of industrial and biological uses.