In the dual challenges of aging and declining birth rates, Taiwan is using the strength of information and communication technology to enhance the services provided by medical institutions. At the same time, the government is actively promoting the intelligence of the medical industry, and major technology companies are also actively cooperating with medical institutions. This study uses 3 eHealth application programs as experimental tools for task design, objectively recording time performance through timing records, and analyzing users’ subjective feelings through system usability scales. The experiment recruited 32 participants and conducted a two-way ANOVA with health literacy as a grouping factor. After experimental analysis, suggestions for the interface design of eHealth application programs are proposed: (1) Avoid presenting information at the same level in multiple columns and rows on the interface, a vertical list is more in line with user reading habits. (2) Icons should avoid using easily confused symbols, which can easily lead to user errors. (3) Using external links for application program functions may bring usability issues for specific user groups in interface operations, it is advisable to avoid using external links. (4) The dropdown menu can be ordered from fewer to more words, avoiding users missing information when searching. (5) There is a significant difference in frustration between eHealth application programs and health literacy.