Negotiation is a common feature of social life, yet past work records a persistent disparity of men profiting more from negotiations than women. Research explaining this difference tends to call on either individual-level differences in men’s and women’s identities and norms or more interactional mechanisms compelled by the activation of differential gender stereotypes and expectations. In this prior work, the gender of negotiators is known, meaning that either of these processes could affect exchange behavior. In the current experiment, the gender of negotiators is not activated leading to the prediction that there should still be differences in exchange behavior and profit if individual-level gender processes are the key driver of the gender negotiation gap. After same- and mixed-gender dyads negotiated for 20 rounds, the results demonstrate that there are no significant differences in the players’ exchange behavior or profit. This indicates the importance of gender saliency in negotiations for activating interactional-level mechanisms that bring about the negotiation gender gap.