Reinforced cement concrete (RCC) structures are prone to corrosion due to the formation of micropores during casting. These micropores develop by entrapping air during the mixing and casting of wet concrete, leading to irregularities in the concrete matrix. This inherent property of cementitious construction compromises the material’s integrity, reducing compressive strength and increasing water absorption, which shortens the service life of the structure. Corrosion is further exacerbated by exposure to moisture and aggressive environmental factors, which penetrate through these pores and cracks. Preventing corrosion in cementitious structures requires sealing the pores and improving the matrix’s durability. Microbially Induced Calcium Carbonate Precipitation (MICCP) has been proposed as a bio-inspired solution for self-healing cementitious materials. In this process, the bacteria mixed in concrete consume nutrients and precipitate calcium carbonate, effectively filling the pores and healing cracks. This microbial activity also creates a protective layer, reducing the ingress of harmful agents like water and chemicals, thereby mitigating corrosion risks. Monitoring the effectiveness of MICCP in preventing corrosion is critical. This study employs non-destructive (NDT) ultrasonic pulse transmission to monitor bacterial self-healing in real-time. The amplitude of the ultrasonic waves passing through the bacterially treated specimen increases as treatment progresses, indicating the gradual closure of pores and defects. The enhanced compressive strength observed in treated specimens over 28 days further confirms the effectiveness of bacterial healing. This non-destructive ultrasonic monitoring approach offers a reliable method for assessing and managing corrosion prevention in cementitious structures and further correlation studies helped to predict the internal health of civil structure non-destructively.