Iron is a highly abundant element, essential for life on earth and integral to various biological processes. It originated from stellar nucleosynthesis, particularly in supernovae type Ia, which produced iron used in planetary formation. Life’s emergence on earth, about 3.5 billion years ago, occurred in an oxygen-free atmosphere, with iron playing a pivotal role in early biochemical reactions. Hydrothermal vents, rich in iron-sulfur (Fe-S) minerals, provided the conditions for prebiotic chemistry and CO2 fixation, potentially driving the synthesis of key organic compounds. Iron-sulfur clusters became crucial cofactors for enzymes involved in energy metabolism, such as ferredoxins, which are ancient electron carriers. Iron’s unique redox properties, allowing electron transfer between ferrous (Fe2+) and ferric (Fe3+) states, enabled its vital role in oxygen transport, cellular respiration, DNA synthesis, replication, and repair, as well as in epigenetic, transcriptional, and translational gene regulatory mechanisms, and in several metabolic pathways. However, the prevalence of insoluble ferric ions under aerobic conditions limits iron’s bioavailability. Moreover, iron’s reactivity poses further challenges, as unshielded iron can undergo redox cycling, leading to oxidative stress. Thus, biological systems have evolved intricate mechanisms to regulate iron acquisition, utilization, and storage. These mechanisms ensure that iron remains bioavailable while preventing toxicity, underscoring its significance throughout the evolution of life.