This chapter explores the applications and significance of ecohydrology in the context of sustainable agricultural water management. Ecohydrology, an interdisciplinary field bridging ecological and hydrological sciences, addresses pressing concerns related to water scarcity, environmental degradation, and ecosystem health in agriculture. By understanding the bidirectional relationship between ecosystems and hydrological processes, ecohydrology offers a holistic approach to optimizing water resources and preserving ecological integrity. The chapter discusses key ecohydrological concepts, approaches in agriculture, and their relevance to sustainable irrigation, ecosystem-based water management, and appropriate land use management. It also highlights the importance of ecohydrological tools such as remote sensing, geographic information systems (GIS), and hydrological models in decision-making processes. Emphasizing ecohydrology’s potential to offer sustainable solutions, the chapter advocates integrating these principles into agricultural water management to create a more resilient and ecologically balanced future.