From the discovery of the gonadal neuroendocrine axis to projects mapping gender/sex diferences in brain and behavior, research in neuroscience has laid the foundation in biosciences for the investigation of sex and, to a lesser degree, gender. Given its role in sex and gender research, neuroscience has also been a central site of critical engagement by feminist science scholars, giving rise to neurofeminism, a subfeld where neuroscience and feminist perspectives on science intersect. To date, neurofeminism has produced critiques, research frameworks, methodologies, epistemologies, and neuroscientifc knowledge that coalesce to advance complex and emancipatory understandings of brain, body, and mind. This chapter aims to demonstrate the instrumental role of neurofeminist research and perspectives in producing alternative operationalizations of sex and gender, particularly with respect to their interrelation. First, a critical overview of dominant and emerging models for investigating sex and gender in neuroscience is provided to highlight benefts of approaching sex and gender as biosocially entangled. Second, the neurofeminist perspective on sex and gender entanglement is further characterized through a series of examples from human and nonhuman animal research. Consideration is then given to potential challenges associated with the neurofeminist approach to entanglement. Finally, the generative potential of neurofeminist science scholarship to improve the science of sex and gender is demonstrated.