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Argania spinosa, an endangered xerophytic tree endemic to southwestern Morocco, plays a crucial role in its native ecosystem and is renowned for its valuable oil with various therapeutic benefits. This study presents a reassembly of the Argania spinosa chloroplast genome using a hybrid sequencing approach that combines Illumina short-read and PacBio long-read technologies. The new assembly reveals a chloroplast genome size of 158,990 base pairs, slightly larger than the previous version, with 79 protein-coding genes. In contrast to the previous assembly, the current version features 4 rRNA genes and 30 tRNA genes. The genome maintains the typical quadripartite structure with two inverted repeats (IRs), a large single-copy (LSC) region, and a small single-copy (SSC) region. Notably, while the previous version included 8 tRNAs with 2 exons each, the new version presents all tRNAs as contiguous sequences. Phylogenetic analysis using 69 conserved protein-coding genes, with Camellia sinensis as the outgroup, reveals well-supported clades within the Sapotaceae family. The new genome assembly enhances the resolution of Argania spinosa genomic data and provides a more accurate basis for evolutionary studies within the family.

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Argan Tree Complete Chloroplast Genome Reconstruction via Hybrid Assembly

  • 会议论文

  • 发表日期：

    2025年

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  • Abdellah Idrissi Azami,
  • Stacy Pirro,
  • Nihal Habib,
  • Douae El Ghoubali,
  • Zainab El Ouafi,
  • Mariyam Khallouqi,
  • Sahar Marchouh,
  • Najib Al Idrissi,
  • Chakib Nejjari,
  • Abdehamid El Mousadik,
  • Hassan Ghazal

摘要

Argania spinosa, an endangered xerophytic tree endemic to southwestern Morocco, plays a crucial role in its native ecosystem and is renowned for its valuable oil with various therapeutic benefits. This study presents a reassembly of the Argania spinosa chloroplast genome using a hybrid sequencing approach that combines Illumina short-read and PacBio long-read technologies. The new assembly reveals a chloroplast genome size of 158,990 base pairs, slightly larger than the previous version, with 79 protein-coding genes. In contrast to the previous assembly, the current version features 4 rRNA genes and 30 tRNA genes. The genome maintains the typical quadripartite structure with two inverted repeats (IRs), a large single-copy (LSC) region, and a small single-copy (SSC) region. Notably, while the previous version included 8 tRNAs with 2 exons each, the new version presents all tRNAs as contiguous sequences. Phylogenetic analysis using 69 conserved protein-coding genes, with Camellia sinensis as the outgroup, reveals well-supported clades within the Sapotaceae family. The new genome assembly enhances the resolution of Argania spinosa genomic data and provides a more accurate basis for evolutionary studies within the family.