Grapevine (Vitis vinifera L.) is a woody, perennial plant, a fruit crop of high economic importance worldwide. Like other plants, it lives in close association with large numbers of microorganisms. Bacteria, fungi and viruses are structured in communities, and each individual can be beneficial, neutral or harmful to the plant. In this sense, grapevines are vulnerable to numerous pathogens, namely the emerging diseases known as grapevine trunk diseases, the bacterial disease, caused by Allorhizobium vitis, grapevine crown gall, posing major threats to the crop’s productivity and quality. With the complex aspect of the management of these biological threats, traditional chemical control methods are often inadequate and environmentally harmful, highlighting the need for sustainable alternatives. This article provides an insight into grapevine crown gall and grapevine trunk diseases, their causative agents, and infection mechanisms. The overall health of grapevines may be significantly influenced by the diversity and structure of microbial communities. This paper explores the potential of biocontrol strategies that utilize beneficial microorganisms as eco-friendly solutions to combat these pervasive diseases. By integrating recent research findings, this review aims to offer insights into effective management practices for enhancing grapevine health and sustainability in viticulture.