The prevalence of microorganisms is a pervasive phenomenon across the Earth’s surface, a substantial proportion of which do not pose any threat to humans. However, there exists a category of microorganisms, referred to as pathogens, that are capable of causing significant diseases. The inherent invisibility of these microorganisms due to their minute size often results in a paucity of awareness regarding their presence within domestic environments. Individuals living in isolation, who may have limited access to disinfectants or cleaning supplies, are particularly susceptible to potentially harmful microorganisms. The project aimed to address these concerns by developing a sensor for detecting air microbiological contamination. A method of cultivation of microorganisms from the outdoor and indoor air was presented. The analysis of microorganisms collected from the air and grown in prepared culture media revealed a preponderance of fungi over bacteria in indoors and bacteria over fungi in outdoors environments. Additionally, a compact 3D-printed incubation chamber was created to maintain temperatures conducive to the growth of human pathogens. The combination of the multi-well culture plate as a microbial contamination detector along with the incubation chamber provides a promising and user-friendly solution for future applications.