Arsenic contamination leads to severe harm to the environment and public health around the globe, therefore, the development of effective and sustainable remediation technology is necessary. Conventional techniques, including physical and chemical methods for arsenic remediation, are often costly and generate enormous secondary waste, requiring complex operation and maintenance. Additionally, they can be less effective under varying environmental conditions and have a high environmental impact as a result of energy consumption and chemical residues. Therefore, this chapter comprehensively explores bioremediation technologies as a promising approach to arsenic removal, leveraging natural biological processes to detoxify contaminated environments. Bioremediation, through microbial and phytoremediation, leads to a cost-effective, environmentally friendly alternative to conventional chemical and physical remediation techniques. The chapter begins with an overview of bioremediation, highlighting the key microbial and plant-based mechanisms involved in the transformation and immobilization of arsenic. Special emphasis is placed on the role of specific potent bacteria, fungi, and hyperaccumulator plants, along with the enzymatic pathways that facilitate arsenic reduction and oxidation. A critical review of current bioremediation technologies, including ‘Bioaugmentation’, ‘Biostimulation’, and ‘Constructed Wetlands’, showcase their efficacy and potential practical viability. Furthermore, the environmental and biological factors influencing the efficacy of bioremediation, such as pH, temperature, redox potential, and dynamics of the microbial community, are elucidated. Future avenues of arsenic bioremediation are explored in bioprocesses that exhibit innovative solutions to mitigate research gaps and proliferate the scalability and viability of these technologies. This chapter, too, critically discusses regulatory and policy measures that are in alignment with the successful adoption of environmentally friendly bioremediation techniques. By the adoption of interdisciplinary perspectives and novel insights, this chapter depicts a new comprehensive insight into bioremediation technologies toward sustainable removal of arsenic, subsequentially contributing towards a healthier and safer environment.