Marine biofouling is a complex community consisting of conditioning film (proteins, bacteria), microfoulers (sponges, tunicates), and macrofoulers (bryozoans, mussels) which are formed on submerged surfaces like ship hulls. Marine biofouling refers to the undesirable growth and adhesion of marine organisms such as barnacles, macro-algae, and microbial slimes on immersed structures. The ship “fouls” with marine life as it travels through the warm tropical waters. Tropical ocean environment teems with microorganisms, of which some adhere, both to the static ship hull and while en route to the destination increasing roughness. LIBS technique can be used to identify and classify biofouling species, and to study their growth. A laboratory-scale LIBS technique was used to analyze biofouling samples and its constituent common water-borne algae and bacterial species. Biofouling samples collected from marine structures like boats and docks were analyzed under lab controlled conditions to identify the different constituents present. Biofouling composition was determined by collecting samples from marine structures and vessels and analyzing them in laboratories. In this study, Laser-Induced Breakdown Spectroscopy (LIBS) in conjunction with Principal Component Analysis (PCA) could identify different types of biofouling organisms by analyzing their spectral signatures, enabling their quick classification and further investigation into their growth patterns. The study showed that the LIBS technique combined with the Principal Component Analysis (PCA) could be used for acquiring the spectra of biofouling species, its rapid classification and studying the growth characteristics.