Green nanosynthesis is increasingly attracting scientists to research and discover. In this study, we have successfully synthesized nano silver from red flesh dragon fruit (Hylocereus polyrhizus) peel extract. The clean and safe nano synthesis can both solve the problem of organic waste in the processing industry and provide silver nanoparticles for different application purposes, especially in antibacterial, antifungal, and medical. The nanoparticles have been morphologically and structurally characterized by modern physicochemical analysis methods: Fourier Transform Infrared Spectroscopy (FT-IR), Dynamic Light Scattering (DLS), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The characteristics of silver nanoparticles are shown on XRD spectrum with angle 2θ = 38.24, 44.28, 64.48, and 77.38. The average nanoparticle size through TEM images was in the range of 20–30 nm. Moreover, silver nanoparticles have been evaluated for their antibacterial ability on Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis, and antioxidant by the ABTS method. The testing process shows that they have very good resistance to oxidation and bacteria. It holds promise for a potential antioxidant and antibacterial application.