Magnetic Resonance Spectroscopy (MRS) is a powerful non-invasive tool for probing the biochemical composition of tissues, with growing applications in both human and animal brain imaging. This chapter provides an overview of static proton (1H)-based MR spectroscopic techniques (both single voxel MRS and multi-voxel MR spectroscopic imaging [MRSI]) for human/rodent brain imaging. This chapter is structured to aid a novice reader grasp key concepts and simultaneously provides details related to fundamental aspects helpful for researchers at all levels. Since the field of MRS/MRSI is broad and rapidly growing, the author has focused on a few selected topics and has provided references to upto-date literature to guide the interested reader to delve deeper into the topics discussed. This chapter begins with providing motivation for the development of MRS and MRSI for applications in vivo, and its utility as a complementary imaging tool with routine MRI. Next, the reader is guided through a few key concepts related to RF pulses in the context of MR spectroscopy, and its use for cubical selection, followed by key technical challenges with MRS/MRSI, preprocessing steps for MRS data, linear combination fitting, and interpretation of data. Finally, a few select key clinical applications of MRS are highlighted, illustrating its utility in diagnosing and monitoring of CNS disorders. This chapter serves as both a practical guide and a conceptual foundation for researchers and clinicians interested in the implementation and interpretation of in vivo brain MRS.