In this chapter, the basic elements of MRI pulse sequence are discussed. Two main pulse sequences that are the basis of most MRI pulse sequences, gradient echo and spin echo, are discussed in more detail. Their diagram, characteristics, and contrast mechanism are described as well as different types of sequences that can be generated by adding or modifying the sequence. For the gradient echo sequence, spoiled gradient echo, ultra-fast gradient echo, steady-state free precession FID, steady-state free precession echo, and balanced steady-state free precession are discussed with their characteristics. At the end of section “Gradient Echo Sequence”, the gradient echo train concept and its application in fast imaging are discussed. For the spic echo sequence, single-echo spin echo, and multi-echo spin echo are described. In addition, Cartesian and non-Cartesian acquisitions, gridding, and two-dimensional versus three-dimensional sequences are discussed in sections “Cartesian and Non-Cartesian Readouts”, “Gridding”, and “Two-Dimensional Versus Three-Dimensional Imaging Sequences”, as well as some of their characteristics and applications.