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In this study, we evaluate the effects of Histone Deacetylase Inhibitor (HDACi), Scriptaid (SCR), and Trichostatin A (TSA) on the preimplantation development and expression of histone H3-lysin 9 (H3K9) acetylation at pronuclear formation and at the 2-cell stage ROSI mouse embryos. The initial experiment demonstrates that the development of ROSI embryos to blastocyst and the quality of ROSI blastocyst was optimal with SCR treatment at 250 nM for 10 h after ROSI oocyte activation. The results of the experiment on the effects of SRC and TSA on the development of ROSI embryos showed that there was no difference between the TSA and SCR treated groups; however, both the TSA and SRC treated groups had a higher rate of ROSI embryos developing to expanded blastocysts as well as higher blastocyst quality than the group without HDACi treatment (25%, 50% and 62%, respectively, P < 0.05). The final experiment showed that the expression of H3K9 acetylation in the pronucleus as well as in the nucleoli of 2-cell ROSI embryos was very low compared to ICSI embryos as well as to the ROSI oocytes treated with TSA or SRC (P < 0.05). Meanwhile, the expression level of H3K9 acetylation was enhanced in both TSA and SRC treated groups and did not differ from the ICSI embryos in 2 cells. From these results, we concluded that enhancing the expression of H3K9 acetylation of ROSI mouse embryos with HDACi within 10 h after activation would increase the development potential of ROSI embryos to blastocysts as well as the cell number of ROSI blastocyst. The results of histone acetylation expression under the effect of TSA and SRC also showed that TSA enhanced histone acetylation expression higher than SCR, however SRC maintained histone acetylation ability longer than TSA.

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Improve the Pre-implantation Developmental Competence of ROSI-Derived Mouse Embryos by Histone Deacetylase Inhibitor

  • 会议论文

  • 发表日期：

    2025年

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  • Pham Xuan Anh,
  • Nguyen Nhat Thinh,
  • Tang Kim Hoang Van,
  • Pham Minh Chien,
  • Bui Hong Thuy,
  • Nguyen Van Thuan

摘要

In this study, we evaluate the effects of Histone Deacetylase Inhibitor (HDACi), Scriptaid (SCR), and Trichostatin A (TSA) on the preimplantation development and expression of histone H3-lysin 9 (H3K9) acetylation at pronuclear formation and at the 2-cell stage ROSI mouse embryos. The initial experiment demonstrates that the development of ROSI embryos to blastocyst and the quality of ROSI blastocyst was optimal with SCR treatment at 250 nM for 10 h after ROSI oocyte activation. The results of the experiment on the effects of SRC and TSA on the development of ROSI embryos showed that there was no difference between the TSA and SCR treated groups; however, both the TSA and SRC treated groups had a higher rate of ROSI embryos developing to expanded blastocysts as well as higher blastocyst quality than the group without HDACi treatment (25%, 50% and 62%, respectively, P < 0.05). The final experiment showed that the expression of H3K9 acetylation in the pronucleus as well as in the nucleoli of 2-cell ROSI embryos was very low compared to ICSI embryos as well as to the ROSI oocytes treated with TSA or SRC (P < 0.05). Meanwhile, the expression level of H3K9 acetylation was enhanced in both TSA and SRC treated groups and did not differ from the ICSI embryos in 2 cells. From these results, we concluded that enhancing the expression of H3K9 acetylation of ROSI mouse embryos with HDACi within 10 h after activation would increase the development potential of ROSI embryos to blastocysts as well as the cell number of ROSI blastocyst. The results of histone acetylation expression under the effect of TSA and SRC also showed that TSA enhanced histone acetylation expression higher than SCR, however SRC maintained histone acetylation ability longer than TSA.