Tissue adhesive hydrogel is an attractive material to develop for diverse purposes across engineering and medical domains, encompassing applications like drug transport, flexible robotics, 3D printing, tissue bonding, and sensor technology. Although numerous tissue adhesives from dopamine have been developed, materials made to mimic the cellular microenvironment should be non-toxic and have appropriate gelation properties, tissue adhesion, and mechanical properties. Here, this study proposes to demonstrate a strategy to modify the self-polymerization hydrogels from dopamine conjugated-hyaluronic acid (DaH) for use in biomedical engineering applications. Dopamine was grafted on HA to form a DaH complex. Different treatments of PBS at different pH levels were used for the formation of the DaH hydrogel in the presence of calcium peroxide. The gelation properties and the rheological properties of the hydrogel were investigated. The biological properties via swelling and degradation behavior were determined. Gelation analysis confirmed the successful formation of dopamine-conjugated hyaluronic acid hydrogel at the investigated treatments. The results showed that the produced DaH hydrogel showed good gelling and tissue adhesive properties. Rheological properties confirm the self-healing properties of the material. Under different conditions, the hydrogels exhibited different physio-biological properties. The biocompatibility of complexes showed a non-toxicity result, thus suggesting a potential tissue adhesive material for tissue engineering applications and drug delivery.