Scholarlink

Freeze-thaw cycles, a process involving repeated freezing and thawing to disrupt cell membranes, are commonly used in tissue decellularization. This study aims to investigate the effects of freeze-thaw cycles on the physicochemical properties of acellular membrane from yellow tuna skin. Scanning electron microscopy, mechanical testing, FTIR spectroscopy, and water absorption tests are employed to characterize the prepared membrane. Results indicate that increasing the freeze-thaw cycles leads to a significant increase in collagen fiber exposure and a decrease in lipid content. The water absorption capacity decreased with increasing freeze-thaw cycles. Compared to traditional chemical-based decellularization methods, the freeze-thaw process offers a milder aproach that preserves the extracellular matrix structure.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effect of Freeze-Thaw Cycles on the Acellular Scaffold Fabricated from Tuna Fish Skin

  • 会议论文

  • 发表日期：

    2025年

查看全文
  • Khoi Minh Le,
  • My-An Tran Le,
  • Thong Lam Vu,
  • Thi Thu Tram Nguyen,
  • An Huyen Lieu,
  • Thi-Hiep Nguyen,
  • Hoan Ngoc Doan

摘要

Freeze-thaw cycles, a process involving repeated freezing and thawing to disrupt cell membranes, are commonly used in tissue decellularization. This study aims to investigate the effects of freeze-thaw cycles on the physicochemical properties of acellular membrane from yellow tuna skin. Scanning electron microscopy, mechanical testing, FTIR spectroscopy, and water absorption tests are employed to characterize the prepared membrane. Results indicate that increasing the freeze-thaw cycles leads to a significant increase in collagen fiber exposure and a decrease in lipid content. The water absorption capacity decreased with increasing freeze-thaw cycles. Compared to traditional chemical-based decellularization methods, the freeze-thaw process offers a milder aproach that preserves the extracellular matrix structure.