Centrifugal spinning, which utilizes centrifugal force to generate polymer jets from a rotating reservoir, is an effective method for nanofiber fabrication technique commonly used to fabricate membranes for biomedical applications. This research focused on combining poly (lactic acid) and chitosan varying concentrations in the presence of glutaraldehyde crosslinker after the centrifugal spinning process to fabricate a hydrophilic membrane for biomedical applications. The influences of chitosan on the resulting membrane's morphology and wettability were explored through scanning electron microscopy and contact angle analysis. The results showed that the concentration of chitosan affected the formation and the membrane morphology. The incorporation of chitosan led to an increase in fiber diameter and reduced the water contact angle of the fabricated membrane. The presence of chitosan significantly reduces the membrane's water contact angle, indicating the potential for use in biomaterials such as wound dressing and scaffold applications.