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Hydrogel-based three-dimensional (3D) matrices have emerged as indispensable tools for cultivating multicellular tumor spheroids (MCTs) as a surrogate in vitro model for investigating spheroid formation dynamics and facilitating drug screening endeavors. Nonetheless, numerous previously utilized hydrogel compositions have proven prohibitively costly and necessitate intricate fabrication procedures, thereby impeding their scalability. In response to these challenges, three innovative hydrogel systems, namely NOCC-OXG, NOCC-AHA, and OXG-CMC, have been synthesized and meticulously tailored to encompass an optimal stiffness range and transparency, thereby augmenting breast cancer progression research, respectively. By subjecting the hydrogel matrices to tailored mechanical stimuli at specific concentrations, cellular attachment and proliferation within the porous scaffolds have been significantly enhanced. Notably, the OXG-CMC with ratio (1:2) hydrogel system demonstrated remarkable efficacy in promoting the morphological and proliferative dynamics of MCF-7 breast cancer cells. These findings underscore the capacity of these novel hydrogel matrices to foster spheroid development and accentuate cell adhesion, thereby furnishing cost-efficient and credible platforms amenable for drug screening endeavors in the realm of cancer research.

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Investigation of Hydrogel-Based 3D Model on the Progression of Multicellular Breast Tumor Spheroids

  • 会议论文

  • 发表日期：

    2025年

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  • Thai Huynh Anh,
  • Thu-La Ngoc Minh,
  • Hai-Nguyen Huu,
  • My-An Tran Le,
  • Hoan Ngoc Doan,
  • Thi-Hiep Nguyen,
  • Toi Van Vo,
  • Han Thi Ngoc To

摘要

Hydrogel-based three-dimensional (3D) matrices have emerged as indispensable tools for cultivating multicellular tumor spheroids (MCTs) as a surrogate in vitro model for investigating spheroid formation dynamics and facilitating drug screening endeavors. Nonetheless, numerous previously utilized hydrogel compositions have proven prohibitively costly and necessitate intricate fabrication procedures, thereby impeding their scalability. In response to these challenges, three innovative hydrogel systems, namely NOCC-OXG, NOCC-AHA, and OXG-CMC, have been synthesized and meticulously tailored to encompass an optimal stiffness range and transparency, thereby augmenting breast cancer progression research, respectively. By subjecting the hydrogel matrices to tailored mechanical stimuli at specific concentrations, cellular attachment and proliferation within the porous scaffolds have been significantly enhanced. Notably, the OXG-CMC with ratio (1:2) hydrogel system demonstrated remarkable efficacy in promoting the morphological and proliferative dynamics of MCF-7 breast cancer cells. These findings underscore the capacity of these novel hydrogel matrices to foster spheroid development and accentuate cell adhesion, thereby furnishing cost-efficient and credible platforms amenable for drug screening endeavors in the realm of cancer research.