The conventional pulse monitoring method of athletes is mainly in the form of pressure sensing, which is affected by the change of position and posture of athletes in the process of long-distance running. The monitored data has errors, which affects the sports monitoring effect of long-distance runners. Therefore, the subject of long-distance runners’ pulse remote monitoring considering mobile wearable sensors is studied. Based on the mobile wearable sensor, the pulse signal characteristics of long-distance runners are extracted, and the pulse signal of operators in the process of movement is monitored according to the pressure of blood flow on the blood vessel wall. Monitor the pulse oxygen saturation of long-distance runners, and obtain the pulse oxygen saturation of long-distance runners with the pulse oxygen measuring instrument, so as to determine the running load state of athletes. Monitor the time domain of periodic changes of human pulse of long-distance runners, analyze the pulse waveform of athletes during long-distance running by using the contraction and expansion state of human heart, and monitor the physiological information of athletes according to the degree of pulse wave reflection and refraction. The simulation experiment verifies that the monitoring method has higher accuracy and can be applied in real life.