Shift work can adversely affect workers’ health in various ways, including sleep disorders, mental and physical health problems, fatigue, and impaired job performance. It has a significant impact on both the physical and mental health of workers due to the disruption of natural circadian rhythms and changes in lifestyle habits. The aim of this study was to assess sleep quality in relation to different work schedules and job roles among hospitality workers. A quantitative, analytical, non-experimental research design was implemented. The Pittsburgh Sleep Quality Index (PSQI) questionnaire was used to assess 83 hospitality workers at a beach in Ecuador. The findings revealed that those engaged in rotating shifts are three times more likely [OR = 3.53 (95% CI = 1.42–8.80)] to experience sleep problems compared to those who do not work rotating shifts. Among the 42 workers on rotating shifts, 30 reported sleep issues. Poor sleep quality was more prevalent among male workers. The productivity of a sector vital to the economy may be compromised if corrective measures are not implemented, potentially leading to workplace accidents or other complications arising from poor sleep quality.