Chemosensors play a vital role in detecting and quantifying various chemical species with high sensitivity and selectivity. This chapter, titled Cyclodextrins for Chemosensing: Principles and Advances, provides a comprehensive exploration of chemosensing, its underlying processes, mechanisms, and the molecular forces that govern sensor performance. Beginning with an overview of chemosensors and the chemosensing process, the chapter elucidates the interplay between sensing and signaling, supported by advanced analytical techniques. A focal point of the chapter is the integration of cyclodextrins into chemosensing systems. Cyclodextrins, with their unique molecular architecture and ability to form inclusion complexes, have emerged as indispensable tools in amplifying sensor performance. The chapter discusses the key contributions of cyclodextrins in chemosensing, comparing cyclodextrin-based chemosensors and cyclodextrin-modified systems. It highlights their versatile roles in enhancing selectivity, sensitivity, and signal amplification, addressing the optimization of chemosensing systems. Additionally, the chapter delves into the detection of specific chemical species, ranging from ions and small molecules to complex biomolecules, and their applications across diverse fields such as environmental monitoring, biomedical diagnostics, and industrial processes. A critical analysis of literature data underscores the advancements in cyclodextrin-based chemosensors, providing insights into current trends and challenges. Concluding with a summary and an overview of the book's content, this chapter serves as a valuable resource for researchers and practitioners, emphasizing the pivotal role of cyclodextrins in advancing the field of chemosensing.