Poly(L-lactide) (PLLA) possesses a high piezoelectric coefficient and electroactive response in the class of biodegradable, bio-based, and biocompatible polymers. Being a Food and Drug Administration (FDA)-approved polymer, the piezoelectric PLLA plays an important role for advances in biomedical applications. The shear piezoelectric coefficient of PLLA involves certain challenges for the piezoelectric characterization and activation. Therefore, a guidance how to characterize and activate the piezoelectric response of shear piezoelectric PLLA concerning practical aspects, such as the piezoelectric mode, geometry, and size is presented. The objective is to enable research community to choose the most adequate piezoelectric activation for PLLA. Furthermore, a comparison with the golden standard normal piezoelectric poly(vinylidene fluoride) (PVDF) will provide better understanding of the piezoelectric behavior of PLLA.