A biosensor is a small analytical instrument or unit that measures one or more analytes by including a biological or biologically derived sensitive recognition element mounted on a physicochemical transducer. On the other hand, microfluidic systems have several advantages over traditional systems. They can reduce the sample and reagent volumes down to nanoliters, which is beneficial for reducing cost and waste. Microfluidic systems can also increase the mixing rate of different reagents, which improves the efficiency of chemical reactions. They allow for high-throughput processing, which means that a large number of samples can be processed quickly. Additionally, microfluidic systems improve transport for controlling flow conditions, which is important for achieving accurate and reproducible results. They also improve detection sensitivity, which helps detect small amounts of analytes. Another advantage of microfluidic systems is that they can be used for both sample preparation and detection, which simplifies the overall process. Because of these benefits, technologies allow biological and chemical components to be combined into a single platform, opening up new possibilities for future biosensing applications such as unprecedented accuracies, real-time detection, simultaneous analysis of multiple analytes in a single device, and portability. The purpose of this study is to highlight recent developments and achievements in microfluidic-based biosensing. The chapter presents examples from published works to illustrate the benefits of combining microfluidic and biosensing technologies, and the flexibility this approach provides for future biosensing applications. Diagnostic microsystems have simplified the process of diagnosing a condition by allowing doctors to pinpoint the exact location of the affected area and track disease progression over time. By using biosensors for multiplexed detection, the analysis throughput can be significantly increased, leading to a greater amount of data acquired in a single test. Microfluidic chips, which are a type of micro-total analysis system (µTAS), have proven to be an essential platform for high-throughput biosensors and portable. While microfluidic chips and biosensors are both powerful tools on their own, their combined capacity is particularly useful for analytical and diagnostic purposes.