The carbon footprint of buildings primarily originates from the production of abiotic building materials like concrete, metals, and plastics, accounting for approximately 14% of global greenhouse gas emissions. Thus, selecting low-carbon materials such as timber becomes increasingly crucial. This paper aims to evaluate the impact of steel-timber composite floor panels on reducing the overall carbon footprint of buildings. Two different structural schemes were compared. The findings indicate that using steel-timber floor panels can decrease the overall carbon footprint by one third compared to traditional reinforced concrete structures. Additionally, while the composite behavior of conventional steelwork and concrete slabs is well-documented and integrated into international building codes, the exploration and standardization of steel-timber composites are still in progress. This study concentrated on the effect of shear connector spacing on the bending behavior of the specimens, with results evaluated in comparison with similar studies. The paper presents the study’s outcomes, revealing that shear connector spacing significantly impacts bending behavior, achieving up to a 48% increase in flexural stiffness normalized by the non-composite stiffness. Furthermore, the moment capacity of composite sections was discovered to be five times greater than that of bare steel sections.