Traffic accidents are a major road safety concern, with human factors, particularly driver fatigue factors, playing a significant role. Driver fatigue is often linked to reduced alertness, slower reaction times, and impaired decision-making. This study focuses on analysis of driver fatigue factors, on an example of the road traffic accident at a section of state road D102 in Croatia. Using the International Road Assessment Programme (iRAP) methodology, the research aims to eliminate road infrastructure as a primary cause of accidents, placing greater focus on human errors produced by driver fatigue. The study also explores how fatigue detection methods can be used to identify human factors contributing to accidents, guiding more effective prevention strategies. A key case study examines a crash involving a young driver that occurred in the early morning hours. The absence of evidence of braking, the youth of the driver, a flat part of road with a monotonous environment and the timing of the accident strongly indicate fatigue as the primary cause. Additionally, the study evaluates the potential safety benefits of reducing speed limits and removing hazardous roadside objects, such as trees and large rocks, to improve road safety. These changes are predicted to reduce the likelihood of fatigue-related incidents, as well as the consequences of traffic accidents caused by fatigue. The study concludes that road safety improvements and other improvements based on scientific analysis of human factors can effectively reduce traffic accidents caused by driver fatigue.