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In scanning optical confocal microscopy, the quality of both lenses, the objective lens and collector lens, is equally responsible for the point spread function. If the object moves along the optical axis, the energy decay versus the defect of focus follows a sinc4 function, as given for the first time by NOMARSKI.

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Basics of Confocal Scanning Laser Microscope

  • 章节

  • 发表日期：

    2025年

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  • Abdallah Mohamed Hamed

摘要

In scanning optical confocal microscopy, the quality of both lenses, the objective lens and collector lens, is equally responsible for the point spread function. If the object moves along the optical axis, the energy decay versus the defect of focus follows a sinc4 function, as given for the first time by NOMARSKI.