According to the benefits of little environmental pollution and cheap combustion cost, nuclear power’s significance in the power grid framework has been growing. However, the power system faces extreme peak shaving pressure when operating nuclear power plants under the typical full power strategy at base load. Therefore, this paper initially delves into the operational dynamics of nuclear power units engaging in daily load following endeavors within the ancillary services market. Meanwhile, the strategic approach for nuclear power plants to participate in the market for ancillary services aimed at maximizing peak shaving revenues is formulated. Secondly, to increase the flexibility and adaptability of the system, a collaborative operating strategy is introduced, involving both pumped storage and nuclear units in peak shaving. This strategy exploits the complementary characteristics of these two technologies. Finally, in order to validate the effectiveness of this approach, a case study is conducted on a regional power system where three different operational scenarios are simulated and comprehensively compared. The results show that the integrated participation of pumped storage and nuclear units in peak load regulation schemes is a more cost-effective solution, underlining the economic viability and technical merits of this collaborative strategy.