Bananas (Musa paradisiaca L.) are a crop of great economic and food importance worldwide. However, its production is affected by various pests and diseases. The use of artisanal biols, organic fertilizers made from natural materials, emerges as a sustainable alternative to promote the growth and development of banana plants. The aim of this study is to analyze the effect of the use of artisanal bioles on the phytochemical stimulation of banana seedlings Musa paradisiaca L., through a bibliometric review and an experimental experiment. An exhaustive bibliometric search was conducted in scientific databases such as Scopus, Web of Science and SciELO, using keywords such as “artisanal bioles”, “banana”, “phytochemical stimulation” and “seedlings”. Relevant scientific articles from the last 10 years were selected and analyzed. In addition, two experimental treatments were established with banana seedlings treated with artisanal bioles and untreated control seedlings. Antioxidant activity, polyphenols and flavonoids were evaluated. The literature review showed that the use of artisanal bioles in banana seedlings has a positive effect on phytochemical stimulation, promoting vegetative growth, increasing the concentration of secondary metabolites. The results of the experimental trial confirmed the findings of the literature review. The use of artisanal bioles in banana seedlings Musa paradisiaca L. is presented as an effective strategy to stimulate the increase of secondary metabolites and healthy development of plants, which translates into a higher yield and quality in production.