Environmental impact and climate change do not recognize national borders. Therefore, collaborating with international partners in this field is paramount. In the twenty-first century, universities have a crucial role in combining teaching and research not only with the transfer of knowledge but also with the renewal of the society. From this perspective, we consider pre-service teachers as agents of positive change. In this article, we present the development and implementation of a virtual and inclusive course for Pre-Service Teachers’ Sustainability Education. In cooperation with five universities across Germany, the Netherlands, and Poland, an online course curriculum was developed to enhance pre-service teachers’ sustainability competences, with a focus on education for sustainable development, globalization, and world citizenship. The course is structured around three key modules: (1) Introduction to the sustainable development issues, (2) World Citizenship, and (3) Globalization. The content of each module was developed and delivered by lecturers from each partner’s university. The paper reports preliminary findings on the aims and expectations following the practical testing of the course with students.