Scholarlink

Anaerobic digestion (AD) transforms organic waste, such as cattle manure, into biogas and anaerobic digestate (ADT), a byproduct often used as a soil conditioner. Microalgae like Chlorella sorokiniana can grow on wastewater, producing biomass suitable for biofuel and animal feed. This study evaluates ADT as a nutrient source for microalgae growth and optimizes biomass production. ADT, derived from cattle manure and whey digestion, was centrifuged and mixed with Inthorn mineral medium in varying ratios (0–18% ADT, 0–81% Inthorn) using a central composite design. Microalgae were cultured in 250 mL flasks at 25 °C under continuous fluorescent light (1.4 klux) and shaken at 150 rpm for 21 days. Growth was monitored by optical density (600 nm) and converted to dry weight concentration (DWC, g/L). Optimal conditions (18% ADT, 40.5% Inthorn) yielded 4.24 g/L DWC, while minimal growth (1.14 g/L) occurred with 9% ADT and no Inthorn. Statistical analysis confirmed most parameters’ significance (p < 0.05) therefore a mathematical model was developed. These results demonstrate that coupling AD with algae cultivation effectively utilizes ADT as a nutrient source, producing valuable biomass and mitigating environmental contamination, though further research is needed to refine ADT concentration.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

An Evaluation and Optimization of Anaerobic Digestate as a Nutrient Source for Microalgae Chlorella Sorokiniana Growth

  • 章节

  • 发表日期：

    2025年

查看全文
  • Marcos Gomes Machado,
  • Robson Junior Nunes Abreu,
  • Geovana Soares Godoy,
  • Kaiquy Duarte de Oliveira,
  • Luis Gustavo Alves de Oliveira,
  • Renato Graça Bauer,
  • Amandha Torres Mendanha,
  • Carla Aparecida da Silva,
  • Jaqueline Cristina Souza,
  • Laura Araújo Ferreira,
  • Guilherme Araújo Silva,
  • Pedro Henrique Estanislau Villar,
  • Suellen Lorraine Siqueira dos Santos,
  • Tainá Cristina Ignácio da Silva,
  • Enio Nazaré de Oliveira Junior

摘要

Anaerobic digestion (AD) transforms organic waste, such as cattle manure, into biogas and anaerobic digestate (ADT), a byproduct often used as a soil conditioner. Microalgae like Chlorella sorokiniana can grow on wastewater, producing biomass suitable for biofuel and animal feed. This study evaluates ADT as a nutrient source for microalgae growth and optimizes biomass production. ADT, derived from cattle manure and whey digestion, was centrifuged and mixed with Inthorn mineral medium in varying ratios (0–18% ADT, 0–81% Inthorn) using a central composite design. Microalgae were cultured in 250 mL flasks at 25 °C under continuous fluorescent light (1.4 klux) and shaken at 150 rpm for 21 days. Growth was monitored by optical density (600 nm) and converted to dry weight concentration (DWC, g/L). Optimal conditions (18% ADT, 40.5% Inthorn) yielded 4.24 g/L DWC, while minimal growth (1.14 g/L) occurred with 9% ADT and no Inthorn. Statistical analysis confirmed most parameters’ significance (p < 0.05) therefore a mathematical model was developed. These results demonstrate that coupling AD with algae cultivation effectively utilizes ADT as a nutrient source, producing valuable biomass and mitigating environmental contamination, though further research is needed to refine ADT concentration.