Scholarlink

Amylase is a hydrolytic enzyme that catalyzes the hydrolysis of starch into reducing sugars and plays a crucial role in the digestion and absorption of nutrients. This enzyme is widely applied in the agricultural industry to improve feed digestibility, especially for young animals with immature digestive systems. This work aimed to study enzyme extract rich in amylase from the fungus Acremonium zeae and apply it to the hydrolysis of animal feed. The amylases produced by the fungus were evaluated for the effect of varying pH and temperature for catalysis, as well as substrate concentration variations, to determine the optimum conditions for their use in biotechnological applications. This study investigated the use of the amylases produced in the hydrolysis of starch present in animal feed consisting of 60% ground corn and 40% soybean meal, to increase the feed's nutritional value. Enzyme activity and reducing sugar production were quantified using UV-VIS spectrophotometry. The best amylase production time was 72 h, with an enzyme activity of 0.270 U/mL, where U is the unit that represents μmol of product release per minute of reaction. In the biochemical characterization, pH 6 and a temperature of 50°C were found to be the optimum parameters for using the enzyme. In the stage of varying the concentration of starch, it was observed that the enzyme follows Michaelis-Menten kinetics and it was possible to calculate the parameters using the Lineweaver-Burk equation, where Km=1.496 mg/mL, which indicated that the amylase produced by Acremonium zeae had a high affinity for the substrate starch. The Vmax value was 0.724 U/mL, which describes the maximum activity that could be achieved by the amylase. When applied to the feed, the enzyme showed a hydrolysis rate of 8.7% of the total mass of the sample, after a seven-day reaction period. This study provides a basis for future research into the amylase produced by Acremonium zeae, highlighting its potential applications in biotechnological processes in the agricultural industry.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biochemical Characterization and Application of Amylase from the Fungus Acremonium zeae in the Hydrolysis of Animal Feed

  • 章节

  • 发表日期：

    2025年

查看全文
  • Esdras Gabriel Rodrigues Sales,
  • Lidia Oliveira Ciriaco Santos,
  • Sthephane Pereira de Oliveira,
  • Luan Ferreira Costa,
  • Pedro Siervi Carrê,
  • Gabriel Mendonça Piazzi,
  • Maíra Nicolau de Almeida

摘要

Amylase is a hydrolytic enzyme that catalyzes the hydrolysis of starch into reducing sugars and plays a crucial role in the digestion and absorption of nutrients. This enzyme is widely applied in the agricultural industry to improve feed digestibility, especially for young animals with immature digestive systems. This work aimed to study enzyme extract rich in amylase from the fungus Acremonium zeae and apply it to the hydrolysis of animal feed. The amylases produced by the fungus were evaluated for the effect of varying pH and temperature for catalysis, as well as substrate concentration variations, to determine the optimum conditions for their use in biotechnological applications. This study investigated the use of the amylases produced in the hydrolysis of starch present in animal feed consisting of 60% ground corn and 40% soybean meal, to increase the feed's nutritional value. Enzyme activity and reducing sugar production were quantified using UV-VIS spectrophotometry. The best amylase production time was 72 h, with an enzyme activity of 0.270 U/mL, where U is the unit that represents μmol of product release per minute of reaction. In the biochemical characterization, pH 6 and a temperature of 50°C were found to be the optimum parameters for using the enzyme. In the stage of varying the concentration of starch, it was observed that the enzyme follows Michaelis-Menten kinetics and it was possible to calculate the parameters using the Lineweaver-Burk equation, where Km=1.496 mg/mL, which indicated that the amylase produced by Acremonium zeae had a high affinity for the substrate starch. The Vmax value was 0.724 U/mL, which describes the maximum activity that could be achieved by the amylase. When applied to the feed, the enzyme showed a hydrolysis rate of 8.7% of the total mass of the sample, after a seven-day reaction period. This study provides a basis for future research into the amylase produced by Acremonium zeae, highlighting its potential applications in biotechnological processes in the agricultural industry.